DNeasy Plant Mini Kit (50)

產(chǎn)品編號：69104

產(chǎn)品名稱：DNeasy Plant Mini Kit (50)

供應(yīng)商：Qiagen

規(guī)格：50T

目錄價：2690

庫存狀態(tài)：現(xiàn)貨

相關(guān)信息：

DNeasy Plant Mini Kit 

植物細(xì)胞，組織或真菌細(xì)胞樣品分離總DNA,最高產(chǎn)量可達 30 µg

純DNA，無污染物和酶抑制劑

快速純化得到即用型DNA

無需有機抽提，無需乙醇沉淀

Format: Mini spin columns 

with 2 ml collection tubes 

Sample source: Plant cells and tissues, fungi 

Sample size: Up to 100 mg wet weight 

Preparation time: <1 hour 

Typical yield:* 3&ndash;30 &micro;g 

Elution volume: 50&ndash;400 &micro;l 

Procedure

Samples are first mechanically disrupted and then chemically lysed. RNA is removed by RNase digestion during lysis. Cell debris is removed and samples are filtered and homogenized by centrifugation through a QIAshredder spin column. Buffering conditions are adjusted, precipitating proteins and polysaccharides, and the lysate is loaded onto the DNeasy Plant spin column. During a brief spin, DNA selectively binds to the silica-gel membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use (see flowchart "DNeasy Plant and DNeasy 96 Plant Procedures"). 

Downstream applications

The DNeasy Plant procedure yields pure nucleic acid, free of polysaccharides and other secondary metabolites often copurified using conventional methods. Such impurities can interfere with spectrophotometric readings and inhibit enzymatic reactions. DNeasy purified DNA is sized up to 40 kb (see figure "Pure DNA (20&ndash;25 kb) for Restriction Analysis"), and is suitable for downstream applications such as: 

PCR (see figure "PCR Analysis of DNA from Different Plant Species")