中文名:凝血酶
英文名:Thrombin
性狀:本品為白色或類白色的凍干塊狀物或粉末
用途:一種由凝血酶前體(血漿中的必要成分)形成的蛋白質(zhì)水解酶,催化纖維蛋白元變成纖維蛋白而促使血液凝固。用于毛細(xì)血管出血的局部止血.
Sigma相關(guān)資料如下:
Sigma T4648 Thrombin from bovine plasma
lyophilized powder, 40-300 NIH units/mg protein (biuret)
Synonym: Factor IIa
CAS Number: 9002-04-4
Enzyme Commission (EC) Number: 3.4.21.5 ( BRENDA | IUBMB )
EC Number: 232-648-7
MDL number: MFCD00166053
Description
Frequently Asked Questions Live Chat and Frequently Asked Questions are available for this Product.
Analysis Note Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Biochem/physiol Actions Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Other Notes View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Preparation Note Activated with bovine brain thromboplastin.
Physical form Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0
Properties
form lyophilized powder
composition Protein, 40-60%
EQP level Premium
storage temp. −20°C