貨號(hào)	產(chǎn)品名稱	廠家	規(guī)格	單價(jià)	詳細(xì)	訂購(gòu)
11088858001	Collagenase D, 100mg	Roche	100 mg	845	詳細(xì)查看	訂購(gòu)
11088882001	Collagenase D, 2.5g, non-steri	Roche	2.5 g	11215	詳細(xì)查看	訂購(gòu)
11088866001	Collagenase D, 500mg	Roche	500 mg	2404	詳細(xì)查看	訂購(gòu)

Application
Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures.
Collagenase D is recommended when functionality and integrity of cell-surface proteins are important.
Product Description
EC 3.4.24.3
Enzyme activity:
Collagenase activity: >0.15 U/mg (according to Wünsch) (25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase D has a normal to high collagenase activity and very low tryptic activity (usually <0.2 units/mg, BAEE as substrate).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Specificity: Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases degrade other proteins as well.
Working concentration: 0.5 - 2.5 mg/ml
Contents
Lyophilizate, non-sterile