
產(chǎn)品編號(hào):539480-100MG
產(chǎn)品名稱:批發(fā)|PROTEINASE K, TRITIRACHIUM ALBUM|蛋白酶K
供應(yīng)商:Merck
規(guī)格:100MG
目錄價(jià):1137
庫存狀態(tài): 現(xiàn)貨
CAS編號(hào):
應(yīng)用范圍:降解蛋白質(zhì)
相關(guān)信息:
特價(jià):480元 另備 SIGMA原裝蛋白酶K 貨號(hào):P6556 規(guī)格:100MG 價(jià)格:1221元
Proteinase K, Tritirachium album
Serine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK.
Product information
Form White lyophilized solid
Formulation Lyophilized from calcium acetate
Molar mass 28500
Specific activity ≥40 mAnsonU/mg protein
Unit definition One unit is defined as the amount of enzyme that will liberate 1.0 µmol tyrosine from casein per min at 37°C, pH 7.5.
Activity ≥30 mAnsonU/mg dry weight
Contaminants DNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C
pH value optimum 7.5 - 12.0
Solubility 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0
EC number 3.4.21.14
CAS number 39450-01-6
Store and ship information
Storage +2°C to +8°C