Cholera Toxin|Cholergen|Cholera enterotoxin|霍亂毒素|
產(chǎn)品編號：C8052
產(chǎn)品名稱：Cholera Toxin|Cholergen|Cholera enterotoxin|霍亂毒素
供應(yīng)商：Sigma
規(guī)格：0.5mg原裝
目錄價：2220
庫存狀態(tài)：大量現(xiàn)貨
CAS編號：9012-63-9 
種屬來源：Cholera Toxin from Vibrio cholerae
相關(guān)信息： 
Cholera Toxin from Vibrio cholerae
別名: Cholera enterotoxin, Cholergen
CAS號: 9012-63-9
MDL number: MFCD00130776
包裝
Package size based on protein content.
Reconstitution
When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, ａnd 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.
Unit Definition
One unit increases adenylate cyclase activity 6-fold, measured as [3H]-cAMP present, using approx. 70,000 CHO cells after incubation for 90 min at 37 °C with [3H]-adenine followed by a 15 min incubation with 1 mM 3-isobutyl-1-methylxanthine （IBMX）.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide ａnd sodium EDTA
Biochem/physiol Actions
Toxin consisting of an A subunit （27 kDa） surrounded by five B subunits （approximately 12 kDa each）, which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein （Gαs）, reducing GTPase activity ａnd activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.
相關(guān)類型 Bacteria, Bioactive Small Molecules, C-CH, Cell Biology, Cell Signaling ａnd Neuroscience,
G Protein Function, G Proteins ａnd Cyclic Nucleotides, Reagents, Toxins ａnd Venoms
Quality Level PREMIUM
assay ~95% （SDS-PAGE）
form lyophilized powder
composition Protein, ~5% Lowry-TCA 
solubility H2O: soluble10 mg/mL
storage temp. 2-8°C