PRESCISSION PROTEASE

產(chǎn)品編號：27-0843-01     

產(chǎn)品名稱：PRESCISSION PROTEASE 

供應(yīng)商：Pharmacia

規(guī)格：500 units

目錄價: 4233

庫存狀態(tài)：現(xiàn)貨

相關(guān)信息：

PreScission Protease

Complete Packsize: 500 units

Application: Used for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors

Unit Definition: One unit will cleave > 90% of 100 μg of a test GST-fusion protein in 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 5°C for 16 h.

Vector: pGEX-6P-1, pGEX-6P-2, pGEX-6P-3

Molecular Weight [Mr]: 46000

Inhibitor: 100 mM ZnCl2 (< 50% inhibition); 100 μM chymostatin; 4 mM Pefabloc

Elution Buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0

Cleavage Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol (DTT), pH 7.0

Supplied As: 2000 units/ml (833 to 1000 units/mg) in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 10 mM EDTA, 1 mM DTT, and 20% glycerol.