Pertussis Toxin (Bordetella pertussis)|百日咳毒素

產(chǎn)品編號：BML-G100-0050     

產(chǎn)品名稱：現(xiàn)貨|Pertussis Toxin (Bordetella pertussis)|百日咳毒素  .0  

供應商：Biomol

規(guī)格：50 ug

目錄價：5358.75

庫存狀態(tài)：現(xiàn)貨

CAS編號：82248-93-9

應用范圍：生化實驗

CAS   號：82248-93-9

純    度：≥99％

外    觀：凍干粉,不易分解,更適合于長期保存.

用途：

         CHO細胞檢測

          血細胞凝集素檢測           

          腺苷酸環(huán)化酶檢測

貯存說明：+4°C環(huán)境下保質期至少一年，溶解后在同樣環(huán)境下保質期至少6個月。請勿凍存！

Pertussis toxin (Bordetella pertussis)

Ultra-pure

BML-G100-0050 

Replaces Prod. #: ALX-630-003

Product Specification

Alternative Name: Islet-activating protein, PTX, Holotoxin

Source: From Bordetella pertussis.

CAS: 82248-93-9

Formulation: Lyophilized. Containing sodium phosphate and sodium chloride. 

Purity: >99%

Purity Detail: This product migrates as five distinct bands when run on a 12% polyacrylamide SDS-urea gel. The five bands consist of one A protomer subunit (S1, MW=26.2 kDa) and four B oligomer subunits (S2, S3, 2x S4, S5; MW’s: 21.9, 21.9, 12.1, 10.9 kDa).

Activity: CHO Cell Assay: The lowest concentration of this preparation at which a positive response (clustered growth pattern) was obtained was 0.1 ng/ml.

Hemagglutination Assay: The lowest concentration of this preparation causing hemagglutination of red blood cells was 312 ng/ml.

Adenylate Cyclase Assay: The adenylate cyclase activity of this preparation is 12.2 picomoles/min/μg in the presence of 1 μM calmodulin.

Hazard: TOXIC.

Long Term Storage: +4°C 

Use/Stability: This preparation is lyophilized, aseptically packaged and sealed under vacuum. Do not vortex. Store at 4°C prior to and following reconstitution. Do not freeze. This toxin is considered to be biologically active. The lyophilized preparation will remain active for at least one year. After reconstitution with sterile water this product should remain active for at least six months if stored at 4°C. Each vial when reconstituted to 500 μl with sterile distilled water, contains 50.0 μg of pertussis toxin in 10 mM sodium phosphate buffer with 50 mM sodium chloride at pH 7.0. The toxin is insoluble and the resulting suspension should be made uniform by gentle mixing, not vortexing, prior to withdrawing aliquots. Do not sterile filter. For use with purified G proteins, the toxin must be structurally converted to artificially create a form of the toxin that interacts directly with G proteins. See Kaslow et al. (1987)

This product should be used only by technically qualif