Biotin RNA Labeling Mix 10x

產(chǎn)品編號(hào):11685597910     

產(chǎn)品名稱(chēng)：Biotin RNA Labeling Mix 10x  

供應(yīng)商：Roche

規(guī)格：40 μl (20 reactions)

目錄價(jià)：1815

庫(kù)存狀態(tài)：大量現(xiàn)貨

相關(guān)信息：

Biotin RNA Labeling Mix 

Application

This labeling mix can be used (with SP6, T3, or T7 RNA polymerase) to add a nonradioactive label to RNA during in vitro transcription. The labeled RNA can easily and safely be detected (e.g ., with a fluorescein-conjugated or enzyme-conjugated streptavidin). 

Product Description

Brief description

The ready-to-use, 10x conc. mix contains:

•ATP, CTP, and GTP, each at a concentration of 10 mM •UTP, 6.5 mM •biotin-16-UTP, 3.5 mMLabeling Efficiency 

A standard labeling reaction with 1 µg linear template DNA and an RNA polymerase produces approx. 10 µg of full-length, biotin-labeled RNA. In a spot test, a combination of anti-biotin-AP and the chemiluminescence substrate CSPD can detect as little as 0.3 pg of the biotin-labeled RNA.

Background Information

The biotin-labeled RNA can be used as a hybridization probe for:

•Northern blots •Southern blots •Plaque or colony lifts •RNase protection experiments •In situ hybridization •Microarray hybridization

Contents

Solution, 10x conc., pH 7.5

保存條件： -20-25度