產(chǎn)品名稱:SS 瓊脂 SS Agar 供應(yīng)商:Oxoid 貨號:CM0099 規(guī)格:500g 產(chǎn)品說明: Directions Suspend 63g in 1 litre of distilled water. Bring to the boil with frequent agitation and allow to simmer gently to dissolve the agar. DO NOT AUTOCLAVE. Cool to about 50°C, mix and pour into sterile Petri dishes. Appearance Dehydrated medium: Straw pink coloured, free-flowing powder Prepared medium: Pink-red coloured gel Storage conditions and Shelf life Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store the prepared medium at 2-8°C. Technique Inoculate the medium heavily with the specimen, spreading a portion of the original inoculum in order to obtain well separated colonies on some part of the plate. Incubate for 18 to 24 hours at 35°C; non-lactose fermenters form colourless colonies, whilst occasional resistant coliforms or other lactose fermenters produce pink or red colonies. In parallel with the SS Agar plate, inoculate a tube of Selenite Broth CM0395 enrichment medium, incubate for 12 hours at 35°C, and sub-culture on to another SS Agar plate. |