Alternate Names / Synonyms: human G-CSF, G-CSF, GCSF, G CSF, h-G-CSF, rh-G-CSF, recombinant human G-CSF, recombinant G-CSF
Gene Symbol: CSF3
Accession #: P09919
Gene ID: 1440
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from 5 mM Sodium Citrate, pH 4.0.
Molecular Weight: 19.7 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 10⁸ units/mg.
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Granulocyte Colony stimulating Factor (G-CSF) is a potent stimulator of bone marrow cells, especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues.
Handling: Centrifuge the vial prior to opening.