NADP/NADPH Quantitation Colorimetric Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- Convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio
Features & Benefits:
• Simple procedure; takes ~2 hours
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio
• Enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH)
Kit components:
• NADP/NADPH Extraction Buffer
• NADP Cycling Buffer
• NADP Cycling Enzyme Mix
• NADPH Developer
• Stop Solution
• NADPH Standard (MW:833.36)
Description:
Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450 nm.
Storage Conditions:
-70°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.