Beta-Secretase Activity Fluorometric Assay Kit
Product Overview
Assay Kit Name:
Beta-Secretase Activity Assay Kit
Kit Summary:
• Detection method- Fluorescence (Ex/Em 335-355/ 495-510 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- Convenient fluorescence method for detecting β-secretase activity in biological and purified samples.
Features & Benefits:
• Simple procedure; takes ~ 1 hour
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable.
Kit components:
• β-Secretase Extraction Buffer
• β-Secretase Reaction Buffer (2X)
• β-Secretase Substrate (in DMSO)
• Active β-Secretase (Lyophilized)
• β-Secretase Inhibitor (in DMSO)
Description:
β-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer’s disease. The β-Secretase activity Assay Kit provides a convenient fluorescence method for detecting β-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.
Storage Conditions:
-20°C
Shipping Conditions:
dry ice
USAGE: For Research Use Only! Not For Use in Humans.