cAMP Direct Immunoassay Kit (Colorimetric)
Product Overview
Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The kit can detect ~0.1-10 pmol/5 μl (or ~ 0.02-2 μM) cAMP samples.
Features & Benefits:
• Simple procedure
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable
• The kit provides a new acetylation procedure to improve detection sensitivity significantly
Kit components:
• 10X cAMP Assay Buffer
• Standard cAMP (10 nmol)
• Neutralizing Buffer
• Acetylating Reagent A
• Acetylating Reagent B
• Rabbit Anti-cAMP pAb
• HRP Developer
• Protein G Coated Plate
Description:
Adenosine 3’,5’-cyclic monophosphate (cyclic AMP, cAMP) is an important “second messenger” involved in many physiological processes. BioVision’s cAMP Assay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cAMP level in biological samples. The kit utilizes recombinant Protein G coated 96-well plate to efficiently anchor cAMP polyclonal antibody on to the plate. cAMP-HRP conjugate directly competes with cAMP from sample binding to the cAMP antibody on the plate. After incubation and washing, the amount of cAMP-HRP bound to the plate can easily be determined by reading HRP activity at OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cAMP in samples. In addition, the kit provides a new acetylation procedure to improve detection sensitivity significantly. The kit can detect ~0.1-10 pmol/5 μl (or ~ 0.02-2 μM) cAMP samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.