cGMP Direct Immunoassay Kit (Colorimetric)
Product Overview
Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.
Features & Benefits:
• Simple procedure
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable
• The kit provides a new acetylation procedure to improve detection sensitivity significantly
Kit components:
• 10X cGMP Assay Buffer
• Standard cGMP (10 nmol)
• Neutralizing Buffer
• Acetylating Reagent A
• Acetylating Reagent B
• Anti-cGMP pAb/BSA
• HRP Developer
• Protein G Coated Plate
Description:
Adenosine and guanosine 3’,5’-cyclic monophosphate (cAMP and cGMP) are important “second messengers” involved in many physiological processes. BioVision’s cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.