Glycogen Colorimetric/Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The assay can detect glycogen 0.0004 to 2 mg/ml.
Features & Benefits:
• Simple procedure; takes ~ 40 minutes
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Glycogen
Kit components:
• Hydrolysis Buffer
• Development Buffer
• OxiRed Probe
• Hydrolysis Enzyme Mix
• Development Enzyme Mix
• Glycogen Standard (2.0 mg/ml)
Description:
Glycogen is the primary short term energy storage molecule in animals, synthesized primarily in the liver and muscle. Glycogen is a branched glucose polymer, in α-1,4 linkage, with branching via α-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. The BioVision Kit is an easy and accurate assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (570 nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.0004 to 2 mg/ml.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.