Ascorbic Acid Colorimetric/Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, food samples, growth medium, etc.
• Species reactivity- Mammalian
• Applications- The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.
Features & Benefits:
• Simple procedure; takes ~ 5 minutes for the reaction to complete
• Fast and convenient
Kit components:
• Ascorbic Acid Assay Buffer
• Ascorbic Acid Probe (lyophilized)
• Dimethylsulfoxide (DMSO, Anhydrous)
• Catalyst
• Ascorbic Acid Enzyme Mix (lyophilized)
• Ascorbic Acid Standard (20 µmole)
Description:
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples. BioVision’s Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples. In this assay, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.