pCR®8⁄GW⁄TOPO® Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers; One Shot® TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink™ Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes. Store all components of the PureLink™ kit at room temperature. Store the One Shot® E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored
Invitrogen’s pCR®8⁄GW⁄TOPO® TA Cloning Kit delivers simple 5 minute cloning, access to the Gateway® recombination cloning vector platform. Our pCR®8 TOPO® TA vector contains a minimal MCS so you sequence more of your insert and less of the vector. And with our patented att sites, the pCR®8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.
The pCR®8⁄GW⁄TOPO® TA Cloning Kit offers:
Fast growing E. coli shortens cloning time
Maximum convenience: includes plasmid miniprep kit
att sites for rapid recombination into any Gateway® destination vector
3´-T overhangs for direct ligation of Taq-amplified PCR products
Sequencing primer sites within 55bp of insertion site ensures you sequence more insert, less vector
Spectinomycin resistance gene for robust selection in E. coli
EcoR I sites flanking the PCR product insertion site for easy excision of inserts