產(chǎn)品名稱：pCR8/GW/TOPO® TA Cloning Kit with One Shot® Mach1™-T1R Chemically Competent E. coli
產(chǎn)品貨號(hào)：K252020
產(chǎn)品包裝：20 reactions
產(chǎn)品價(jià)格：4395
Invitrogen’s pCR8⁄GW⁄TOPO® TA Cloning Kit delivers fast, efficient and reliable cloning. Additionally, this vector is ideal for use in Gateway® recombination cloning into various destination vectors for a multitude of applications. This pCR®8 cloning kit offers the following feature Fast 5 minute TOPO® TA Cloning delivers 95% recombinants
3´-T overhangs simplify cloning of Taq-amplified PCR products
Fast growing MachI cells shorten cloning time
Compatible with Gateway® recombination cloning enabling access to a multitude of destination vectors
Sequencing primer sites located close to cloning insertion site so you sequence more insert, less vector the first time.
Spectinomycin resistance gene for robust selection in E. coli
EcoR I sites flanking the PCR product insertion site for easy excision of inserts
The pCR®8⁄GW⁄TOPO® TA Cloning Kit contains the TOPO®-adapted, ready-to-use pCR®8⁄GW⁄TOPO® Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot® Mach1™-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot® E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored
Regulatory Statement: For Research Use Only. Not for any animal or human therapeutic or diagnostic use.
Vector Type: pCR
Product Size: 20 reactions
Cloning Method: TOPO® with Gateway®