His.Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods His-Tag融合蛋白可以在變性條件下純化，以下是基本的路線。包含體用6M鹽酸胍或8M的尿素buffer加BME溶解，親和樹脂用同樣的buffer平衡，上樣后用同樣的buffer加10mM的咪唑洗雜。最后洗脫可采用同樣的buffer加200mM的咪唑洗脫或用pH4.5的8M尿素洗脫。上樣和洗雜的buffer的pH至少要在7以上 。如果洗脫的蛋白還不夠純，可以進(jìn)一步提高咪唑的濃度