69671  Thrombin, Restriction Grade
Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.
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Product information 
Molar mass  37,000 
Avoid freeze/thaw  Yes 
Unit definition  One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl2, 50 µg fusion protein. 
Components  • 50 U Thrombin
• 1 ml 10X Thrombin Cleavage Buffer
• 2 ml 1X Thrombin Dilution/Storage Buffer
• 10 µg Cleavage Control Protein
Source  Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. 
RTECS  XO8950000 
CAS number  9002-04-4 
Store and ship information 
Storage  -20°C 
Ship  Shipped with Blue Ice or with Dry Ice
Multiple Toxicity Values, refer to MSDS