Zeocin™
InvivoGen is the sole worldwide producer of Zeocin™, a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells).
Resistance to Zeocin™ is conferred by the Sh ble gene product, which inactivates Zeocin™ by binding to the antibiotic. Sh ble is a small gene, only 370bp in size, carried in a number of InvivoGen’s vectors.
Zeocin™ is a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin™ causes cell death by intercalating into DNA and cleaving it. The action of Zeocin™ is effective on most aerobic cells.
Working concentration:
- 50-400 µg/ml in mammalian cells
- 25 µg/ml in bacteria
CAS number: 11006-33-0
Purity: controlled by HPLC >90%
Molecular weight: 1525
Formula: C55H85O21N20S2Cu - HCl
Quality control
Zeocin™ in solution in HEPES buffer, pH 7.25, is filtered to sterility for customer convenience, and validated for cell-culture usage.
Activity controlled by microbiological assays