Zeocin
InvivoGen is the sole worldwide producer of Zeocin, a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990.
Zeocin causes cell death by intercalating into DNA and cleaving it. This antibiotic is effective on most aerobic cells and is therefore useful for selection in bacteria, eukaryotic microorganisms, plant and animal cells. Resistance to Zeocin™ is conferred by the Sh ble gene product which inactivates Zeocin™ by binding to the antibiotic.
Zeocin is used at a concentration of 50-300 µg/ml for selection in mammalian cells and 25 µg/ml for bacterial selection.
Working concentration:
50-300 µg/ml in mammalian cells
25 µg/ml in bacteria
CAS number: 11006-33-0
Purity: >90%
Molecular weight: 1525
Formula: C55H85O21N20S2Cu - HCl