Poly(I:C) (HMW) Fluorescein
Fluorescein-labeled poly(I:C) (HMW)
Poly(I:C) Fluorescein was chemically labeled by covalent coupling of a fluorescein probe containing a reactive alkylating group. This confers fluorescent properties to poly(I:C) (HMW) whilst retaining TLR3 recognition properties.
Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection. Both natural and synthetic dsRNAs are known to induce type I interferons (IFN) and other cytokines production. Poly(I:C) is recognized by Toll-like receptor 3 (TLR3).
Specificity: TLR3 agonist
Working Concentration: 10 ng - 10 μg/ml
Endotoxin level: EndoFit™ (<0.001 EU/μg)
Appearance: Pale yellow lyophilized powder
Spectral Properties of Fluorescein
Excitation λ max: 492 nm
Emission λ max: 518 nm