原裝正品|GenMute siRNA Transfection Reagent for Neuro2A Cell
Description:
GenMute™ siRNA Transfection Reagent for Neuro-2A Cell is pre-optimized for transfecting siRNA to Neuro-2A cells. Refer to the following optimal transfection conditions for maximal silencing on Neuro-2A cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Cell culture medium     DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of transfection  ~50%
Optimal siRNA concentration    30 nM
Diluent for siRNA and GenMute™ Reagent   1xGenMute™ Transfection Buffer
Incubation Time to Form GenMute™/siRNA Complex  ~15 minutes at RT
Maximal Silencing Efficiency     48 hours
Transfection Results:
Reporter Gene   Renilla Luciferase 
Silencing Efficiency (% ) 90%
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.