原裝正品|GenMute siRNA Transfection Reagent for MEFs
Description:
GenMute™ siRNA Transfection Reagent for MEFs is pre-optimized for transfecting siRNA to Mouse Embryonic Fibroblasts (MEFs). MEFs are required to support the growth of undifferentiated mouse or human ES cells, iPS cells, thus they are also called feeder cells. MEFs are isolated from mouse embryos and are used at their early passages. Before MEFs are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing.
Summary of Optimal Transfection Conditions:
Cell culture medium     DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of transfection  ~50%
Optimal siRNA concentration    40 nM
Diluent for siRNA and GenMute™ Reagent   1xGenMute™ Transfection Buffer
Incubation Time to Form GenMute™/siRNA Complex  ~15 minutes at RT
Maximal Silencing Efficiency     48 hours
Transfection Results:
Reporter Gene   Renilla Luciferase 
Silencing Efficiency (% ) 90%
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.