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TdT FragEL TM DNA Fragment. Detect. Kit

發(fā)布人:浩然生物 瀏覽 4028次【字號(hào) 】 發(fā)布時(shí)間:2011年08月22日 打印本頁(yè)
貨號(hào) 產(chǎn)品名稱 廠家 規(guī)格 單價(jià) 詳細(xì) 訂購(gòu)
QIA33-1EA TDT FRAGEL TM DNA FRAGMENT. DETECT. KIT Merck *** 5849.44320712695 詳細(xì)查看 訂購(gòu)

檢測(cè)原理:
通過(guò)DNA 片段末端標(biāo)記(Fragment End Labeling, FragELTM)檢測(cè)凋亡細(xì)胞的DNA 片段。細(xì)胞凋亡中,
染色體DNA 雙鏈斷裂或單鏈斷裂而產(chǎn)生大量的粘性3'-OH 末端,可在脫氧核糖核苷酸末端轉(zhuǎn)移酶
(TdT)的作用下,將生物素標(biāo)記和未標(biāo)記的脫氧核糖核苷酸標(biāo)記到DNA 的3'-末端,生物素標(biāo)記的脫
氧核糖核酸可以通過(guò)Streptavidin-HRP 進(jìn)行檢測(cè),通過(guò)DAB 顯色,從而檢測(cè)到凋亡細(xì)胞DNA 片段。由
于正常的或正在增殖的細(xì)胞幾乎沒(méi)有DNA 的斷裂,因而沒(méi)有3'-OH 形成,很少能夠被染色。以甲基綠
復(fù)染,便于從形態(tài)學(xué)上分辨評(píng)估正常細(xì)胞和凋亡細(xì)胞。
Product information 
Format:  Microscopy 
Form:  50 Tests 
Detection method:  Colorimetric 
Species reactivity:  a broad range of species 
Applications:  Frozen Sections
Paraffin Sections 
Assay time  :4 h 
Sample type : Frozen or paraffin sections or fixed-cell preparations 
Kit contains : Counterstain, Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Stop Buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H2O2/Urea Tablets, Methyl Green Counterstain, and a user protocol. 
Storage  -20°C 
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS 
Safety information 
S Phrase  S: 26-36/37/39-45-53
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Avoid exposure - obtain special instructions before use. 
R Phrase  R: 23/25-36/37/38-42/43-45-61
Toxic by inhalation and if swallowed.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
May cause cancer.


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