檢測(cè)原理:
通過(guò)DNA 片段末端標(biāo)記(Fragment End Labeling, FragELTM)檢測(cè)凋亡細(xì)胞的DNA 片段。細(xì)胞凋亡中,
染色體DNA 雙鏈斷裂或單鏈斷裂而產(chǎn)生大量的粘性3'-OH 末端,可在脫氧核糖核苷酸末端轉(zhuǎn)移酶
(TdT)的作用下,將生物素標(biāo)記和未標(biāo)記的脫氧核糖核苷酸標(biāo)記到DNA 的3'-末端,生物素標(biāo)記的脫
氧核糖核酸可以通過(guò)Streptavidin-HRP 進(jìn)行檢測(cè),通過(guò)DAB 顯色,從而檢測(cè)到凋亡細(xì)胞DNA 片段。由
于正常的或正在增殖的細(xì)胞幾乎沒(méi)有DNA 的斷裂,因而沒(méi)有3'-OH 形成,很少能夠被染色。以甲基綠
復(fù)染,便于從形態(tài)學(xué)上分辨評(píng)估正常細(xì)胞和凋亡細(xì)胞。
Product information
Format: Microscopy
Form: 50 Tests
Detection method: Colorimetric
Species reactivity: a broad range of species
Applications: Frozen Sections
Paraffin Sections
Assay time :4 h
Sample type : Frozen or paraffin sections or fixed-cell preparations
Kit contains : Counterstain, Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Stop Buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H2O2/Urea Tablets, Methyl Green Counterstain, and a user protocol.
Storage -20°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Safety information
S Phrase S: 26-36/37/39-45-53
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Avoid exposure - obtain special instructions before use.
R Phrase R: 23/25-36/37/38-42/43-45-61
Toxic by inhalation and if swallowed.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
May cause cancer.