688000 Y-27632 ( Y-27632 )
ROCK Inhibitor, (R)-(+)-trans-N-(4-Pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide, 2HCl
A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (Ki = 140 nM for p160ROCK). Also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP. Exhibits 10- to 50-fold lower affinity for PKCε than p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at higher concentrations (~100 µM). Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contraction. Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency. A 5 mM (500 µg/296 µl) solution of Y-27632 (Cat. No. 688001) in H2O is also available.
產(chǎn)品信息
Form White solid
Primary Target p160ROCK
Primary Target Ki 140 nM
Molar mass 338.3
Protect from Light Yes
Hygrocopic Yes
Packaged under inert gas Yes
Cell permeable Yes
ATP Competitive Yes
Purity ≥95% by HPLC
Solubility H2O
Chemical formula C14H21N3O • 2HCl • H2O