品名: 5´ RACE System for Rapid Amplification of cDNA Ends, Version 2.0
Cat. No. 18374-058
Product Size: 10 reactions
產(chǎn)品介紹:
The 5´ RACE System, Version 2.0 is suitable for rapid amplification of cDNA ends (RACE) (1) and anchored PCR (2) between a defined point in the mRNA and the 5´ end. The system is useful for the amplification of rare messages for which little sequence information may be available, and for capturing the 5´ end information of mRNA (3,4). The 5´ RACE system:
Uses the advanced capabilities of SuperScript® II RNase H- RT for greater first-strand cDNA yields and increased cDNA lengths
Prepares specific cDNA from up to 1 µg of total or poly(A)+ RNA in one reaction
Offers improved first-strand cDNA purification procedure that yields cDNA suitable for tailing
Uses recombinant TdT with optimized reaction conditions in a PCR-compatible buffer to provide an efficient primer annealing site for PCR (Figure 1)
Contents & Storage:
The 5´ RACE System is supplied with the components listed in Table 1. Store the reagents at -20°C and the DNA purification components at +4°C. All reagents guaranteed stable for 6 months when properly stored.